Immunohistochemistry (IHC) involves the specific binding of antibodies to antigens in biological tissues. This technique is a powerful tool for analyzing the expression, localization, and distribution of specific proteins within tissue cells. Given that the unique protein expression patterns often correlate with specific diseases or cellular events, IHC staining finds extensive applications in clinical diagnosis and basic research, including pathway studies and biomarker development.
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In IHC, proteins are observed under a brightfield microscope by employing enzymatic markers, like DAB chromogen, to highlight the linked proteins. This technique is primarily used for the identification of individual targets.
In IHC-IF, antibodies that are bound to their respective proteins are conjugated with a fluorophore or fluorescent dye. To observe these proteins, a fluorescent microscope is used. Multiplex immunofluorescence, a subset of IF, utilizes various markers and wavelengths to detect multiple proteins with a single tissue sample.
Through a collaboration with a third-party company, we have validated a large selection of antibodies across multiple different species that are specific to commonly used IHC staining targets. Our team of experienced histologists can also assist in validating your chosen antibodies and deliver the conclusive IHC results you desire.